PrimePCR™ Probe Assay: DFFB, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053908
Assay Design:   exonic
Chromosome Location:   1:3775304-3775396question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000422171 ENST00000372413 ENST00000372414 ENST00000414879 ENST00000429895 ENST00000438120 ENST00000359947 ENST00000348729 ENST00000353963 ENST00000381894 ENST00000268756 ENST00000310123 ENST00000392225 ENST00000555697 ENST00000556585 ENST00000418511 ENST00000335108 ENST00000378209 ENST00000338895 ENST00000378212 ENST00000341385 ENST00000380056 ENST00000380059 ENST00000437836 ENST00000539553 ENST00000406610 ENST00000462231 ENST00000351153 ENST00000349953 ENST00000462833 ENST00000491190 ENST

Gene Information

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   DFFB
Gene Name:   DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
Aliases:   CAD, CPAN, DFF-40, DFF2, DFF40
RefSeq:   NC_000001.10 NT_004350.19
Ensembl:   ENSG00000169598
Entrez:   1677
UniGene:   Hs.133089
Chromosome Mapping:   1p36.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.660000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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