PrimePCR™ Probe Assay: CXCL10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053880
Assay Design:   exonic
Chromosome Location:   4:76942876-76943023question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000306602

Gene Information

This gene encodes a chemokine of the CXC subfamily and ligand for the receptor CXCR3. Binding of this protein to CXCR3 results in pleiotropic effects including stimulation of monocytes natural killer and T-cell migration and modulation of adhesion molecule expression. [provided by RefSeq Jul 2008]

Gene Symbol:   CXCL10
Gene Name:   chemokine (C-X-C motif) ligand 10
Aliases:   C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000169245
Entrez:   3627
UniGene:   Hs.632586
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997600
y-intercept 37.640000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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