PrimePCR™ Probe Assay: CTRB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053850
Assay Design:   exonic
Chromosome Location:   16:75258622-75258756question
Amplicon Length:   105
Splice Variants Targeted:   ENST00000593290 ENST00000373093 ENST00000303037 ENST00000562106 ENST00000567767 ENST00000562387 ENST00000361017

Gene Information

The protein encoded by this gene is one of a family of serine proteases that is secreted into the gastrointestinal tract as an inactive precursor which is activated by proteolytic cleavage with trypsin. [provided by RefSeq Oct 2011]

Gene Symbol:   CTRB1
Gene Name:   chymotrypsinogen B1
Aliases:   CTRB, FLJ42412, MGC88037
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000168925
Entrez:   1504
UniGene:   Hs.610926
Chromosome Mapping:   16q23.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.900000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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