PrimePCR™ Probe Assay: ADAM9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ADAM9, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ADAM9, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053826
Assay Design:   exonic
Chromosome Location:   8:38854521-38854632question
Amplicon Length:   82
Splice Variants Targeted:   ENST00000472257 ENST00000497477 ENST00000460329 ENST00000483466 ENST00000463103 ENST00000402939 ENST00000330909 ENST00000371030 ENST00000428977 ENST00000429976 ENST00000301807 ENST00000466936 ENST00000481513 ENST00000487273

Gene Information

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions including fertilization muscle development and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins binds mitotic arrest deficient 2 beta protein and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq Jul 2010]

Gene Symbol:   ADAM9
Gene Name:   ADAM metallopeptidase domain 9
Aliases:   CORD9, KIAA0021, MCMP, MDC9, Mltng
RefSeq:   NC_000008.10 NG_016335.1 NT_167187.1
Ensembl:   ENSG00000168615
Entrez:   8754
Chromosome Mapping:   8p11.22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.940000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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