PrimePCR™ Probe Assay: ECI1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: ECI1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: ECI1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053771
Assay Design:   exonic
Chromosome Location:   16:2294460-2296860question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000282605 ENST00000361895 ENST00000402835 ENST00000395003 ENST00000261722 ENST00000535348 ENST00000535359 ENST00000218104 ENST00000361971 ENST00000538776 ENST00000538966 ENST00000301729 ENST00000562238 ENST00000570258 ENST00000566379 ENST00000599531 ENST00000543388 ENST00000479991 ENST00000545866 ENST00000263674 ENST00000309083 ENST00000601366 ENST00000596875 ENST00000593339 ENST00000598748

Gene Information

This gene encodes a member of the hydratase/isomerase superfamily. The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids. It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis- mono- and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates. Alternatively spliced transcript variants have been described. [provided by RefSeq May 2010]

Gene Symbol:   ECI1
Gene Name:   enoyl-CoA delta isomerase 1
Aliases:   DCI
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000167969
Entrez:   1632
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.950000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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