PrimePCR™ Probe Assay: GATAD2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053719
Assay Design:   exonic
Chromosome Location:   19:19496942-19576273question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000417582 ENST00000457895 ENST00000432704 ENST00000404158 ENST00000429242 ENST00000358713 ENST00000444839 ENST00000360315 ENST00000252577 ENST00000537887

Gene Information

Description Not Available

Gene Symbol:   GATAD2A
Gene Name:   GATA zinc finger domain containing 2A
Aliases:   FLJ20085, FLJ21017, p66alpha
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000167491
Entrez:   54815
UniGene:   Hs.118964
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 35.730000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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