PrimePCR™ Probe Assay: GPRC5B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053701
Assay Design:   exonic
Chromosome Location:   16:19884016-19884105question
Amplicon Length:   60
Splice Variants Targeted:   ENST00000297293 ENST00000300571 ENST00000535671 ENST00000569479 ENST00000569847 ENST00000566822 ENST00000562469 ENST00000568214 ENST00000570142 ENST00000537135 ENST00000240587 ENST00000296125

Gene Information

The protein encoded by this gene is a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The specific function of this protein is unknown; however this protein may mediate the cellular effects of retinoic acid on the G protein signal transduction cascade. [provided by RefSeq Jul 2008]

Gene Symbol:   GPRC5B
Gene Name:   G protein-coupled receptor, family C, group 5, member B
Aliases:   RAIG-2, RAIG2
RefSeq:   NC_000016.9 NT_010393.16
Ensembl:   ENSG00000167191
Entrez:   51704
UniGene:   Hs.148685
Chromosome Mapping:   16p12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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