PrimePCR™ Probe Assay: B4GALNT2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053695
Assay Design:   exonic
Chromosome Location:   17:47247043-47247164question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000504681 ENST00000393354 ENST00000300404

Gene Information

B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-14 linkage to a subterminal galactose residue substituted with an alpha-23-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al. 2003 [PubMed 12678917]).[supplied by OMIM Mar 2008]

Gene Symbol:   B4GALNT2
Gene Name:   beta-1,4-N-acetyl-galactosaminyl transferase 2
Aliases:   B4GALT, GALGT2, MGC142235, MGC142237
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000167080
Entrez:   124872
UniGene:   Hs.374679
Chromosome Mapping:   17q21.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.410000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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