PrimePCR™ Probe Assay: PLIN1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053667
Assay Design:   exonic
Chromosome Location:   15:90208011-90208146question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000300055 ENST00000560330 ENST00000430628

Gene Information

The protein encoded by this gene coats lipid storage droplets in adipocytes thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and when unphosphorylated may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR but encoding the same protein have been found for this gene. [provided by RefSeq Feb 2009]

Gene Symbol:   PLIN1
Gene Name:   perilipin 1
Aliases:   PERI, PLIN
RefSeq:   NC_000015.9 NT_010274.17
Ensembl:   ENSG00000166819
Entrez:   5346
UniGene:   Hs.103253
Chromosome Mapping:   15q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.810000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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