PrimePCR™ Probe Assay: ZMAT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053642
Assay Design:   exonic
Chromosome Location:   X:101138707-101138830question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000372782 ENST00000540921 ENST00000458570

Gene Information

This gene encodes a protein containing Cys2-His2 (C2H2)-type zinc fingers which are similar to those found in the nuclear matrix protein matrin 3. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Jan 2012]

Gene Symbol:   ZMAT1
Gene Name:   zinc finger, matrin-type 1
Aliases:   KIAA1789, MGC176597, MGC176728
RefSeq:   NC_000023.10 NG_016330.1 NT_011651.17
Ensembl:   ENSG00000166432
Entrez:   84460
UniGene:   Hs.496512
Chromosome Mapping:   Xq21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.610000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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