PrimePCR™ Probe Assay: TPTE, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053618
Assay Design:   exonic
Chromosome Location:   21:10906789-10906947question
Amplicon Length:   129
Splice Variants Targeted:   ENST00000298232 ENST00000361285 ENST00000342420

Gene Information

Description Not Available

Gene Symbol:   TPTE
Gene Name:   transmembrane phosphatase with tensin homology
Aliases:   CT44, PTEN2
RefSeq:   NC_000021.8 NT_029490.4
Ensembl:   ENSG00000166157
Entrez:   7179
Chromosome Mapping:   21p11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 37.410000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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