PrimePCR™ Probe Assay: GPR176, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053610
Assay Design:   exonic
Chromosome Location:   15:40094116-40094207question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000299092 ENST00000561100 ENST00000543580

Gene Information

Members of the G protein-coupled receptor family such as GPR176 are cell surface receptors involved in responses to hormones growth factors and neurotransmitters (Hata et al. 1995 [PubMed 7893747]).[supplied by OMIM Jul 2008]

Gene Symbol:   GPR176
Gene Name:   G protein-coupled receptor 176
Aliases:   GPR, Gm1012
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000166073
Entrez:   11245
UniGene:   Hs.37196
Chromosome Mapping:   15q14-q15.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 35.900000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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