PrimePCR™ Probe Assay: NAA15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053443
Assay Design:   exonic
Chromosome Location:   4:140311054-140311202question
Amplicon Length:   119
Splice Variants Targeted:   ENST00000296543 ENST00000398947

Gene Information

This gene encodes a protein of unknown function. However similarity to proteins in yeast and other species suggests that this protein may be an N-acetyltransferase. [provided by RefSeq Jul 2008]

Gene Symbol:   NAA15
Gene Name:   N(alpha)-acetyltransferase 15, NatA auxiliary subunit
Aliases:   Ga19, NARG1, NATH, TBDN100
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000164134
Entrez:   80155
UniGene:   Hs.732391
Chromosome Mapping:   4q31.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.860000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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