PrimePCR™ Probe Assay: BSN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053426
Assay Design:   exonic
Chromosome Location:   3:49693656-49693802question
Amplicon Length:   117
Splice Variants Targeted:   ENST00000296452

Gene Information

Neurotransmitters are released from a specific site in the axon terminal called the active zone which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq Jul 2008]

Gene Symbol:   BSN
Gene Name:   bassoon (presynaptic cytomatrix protein)
Aliases:   ZNF231
RefSeq:   NC_000003.11 NG_011438.1 NG_015892.1 NT_022517.18
Ensembl:   ENSG00000164061
Entrez:   8927
UniGene:   Hs.194684
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 36.280000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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