PrimePCR™ Probe Assay: CLEC3B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053399
Assay Design:   exonic
Chromosome Location:   3:45067870-45067961question
Amplicon Length:   62
Splice Variants Targeted:   ENST00000455860 ENST00000436311 ENST00000487664 ENST00000371757 ENST00000486577 ENST00000491806 ENST00000488444 ENST00000490355 ENST00000298480 ENST00000263604 ENST00000399344 ENST00000383194 ENST00000296130 ENST00000436535 ENST00000400335 ENST00000400338 ENST00000525144 ENST00000529097 ENST00000416561 ENST00000377184 ENST00000269095 ENST00000461854 ENST00000520305 ENST00000523501 ENST00000536246 ENST00000409021 ENST00000409371 ENST00000266000 ENST00000374542 ENST00000414083 ENST00000399060 ENST

Gene Information

Description Not Available

Gene Symbol:   CLEC3B
Gene Name:   C-type lectin domain family 3, member B
Aliases:   DKFZp686H17246, TN, TNA
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000163815
Entrez:   7123
UniGene:   Hs.476092
Chromosome Mapping:   3p22-p21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996800
y-intercept 35.670000
Efficiency 106

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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