This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: STT3B, Human
PrimePCR™ Template for Probe Assay: STT3B, Human
The SIMP protein contains a highly immunogenic minor histocompatibility antigen epitope of 9 amino acids B6(dom1). Like ITM1 (MIM 601134) SIMP is homologous to yeast STT3 an oligosaccharyltransferase essential for cell proliferation (McBride et al. 2002 [PubMed 12439619]).[supplied by OMIM Mar 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.