PrimePCR™ Probe Assay: IVL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053315
Assay Design:   exonic
Chromosome Location:   1:152884247-152884361question
Amplicon Length:   85
Splice Variants Targeted:   ENST00000368764 ENST00000392667

Gene Information

Involucrin a component of the keratinocyte crosslinked envelope is found in the cytoplasm and crosslinked to membrane proteins by transglutaminase. This gene is mapped to 1q21 among calpactin I light chain trichohyalin profillaggrin loricrin and calcyclin. [provided by RefSeq Jul 2008]

Gene Symbol:   IVL
Gene Name:   involucrin
Aliases:   Not Available
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000163207
Entrez:   3713
UniGene:   Hs.516439
Chromosome Mapping:   1q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.110000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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