PrimePCR™ Probe Assay: FAIM3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053284
Assay Design:   exonic
Chromosome Location:   1:207086305-207087113question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000367091 ENST00000442471 ENST00000525793 ENST00000529560 ENST00000420007 ENST00000559476 ENST00000446923

Gene Information

Fc receptors specifically bind to the Fc region of immunoglobulins (Igs) to mediate the unique functions of each Ig class. FAIM3 encodes an Fc receptor for IgM (see MIM 147020) (Kubagawa et al. 2009 [PubMed 19858324]; Shima et al. 2010 [PubMed 20042454]).[supplied by OMIM Jul 2010]

Gene Symbol:   FAIM3
Gene Name:   Fas apoptotic inhibitory molecule 3
Aliases:   FCMR, TOSO
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000162894
Entrez:   9214
UniGene:   Hs.58831
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.840000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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