PrimePCR™ Probe Assay: ADCY9, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053227
Assay Design:   exonic
Chromosome Location:   16:4163904-4164023question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000294016 ENST00000572288

Gene Information

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors protein kinases and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin calcium and somatostatin. [provided by RefSeq Jul 2008]

Gene Symbol:   ADCY9
Gene Name:   adenylate cyclase 9
Aliases:   AC9, FLJ12046
RefSeq:   NC_000016.9 NG_011434.1 NT_010393.16
Ensembl:   ENSG00000162104
Entrez:   115
UniGene:   Hs.391860
Chromosome Mapping:   16p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.940000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download