PrimePCR™ Probe Assay: JOSD2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053206
Assay Design:   exonic
Chromosome Location:   19:51009399-51009491question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000391815 ENST00000595669 ENST00000598418 ENST00000601423 ENST00000400358 ENST00000400359 ENST00000403586 ENST00000382932 ENST00000315765 ENST00000343544

Gene Information

Description Not Available

Gene Symbol:   JOSD2
Gene Name:   Josephin domain containing 2
Aliases:   FLJ29018
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000161677
Entrez:   126119
UniGene:   Hs.467151
Chromosome Mapping:   19q13.33

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.140000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download