PrimePCR™ Probe Assay: FCER1G, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053096
Assay Design:   exonic
Chromosome Location:   1:161188824-161188933question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000289902

Gene Information

The high affinity IgE receptor is a key molecule involved in allergic reactions. It is a tetramer composed of 1 alpha 1 beta and 2 gamma chains. The gamma chains are also subunits of other Fc receptors. [provided by RefSeq Jul 2008]

Gene Symbol:   FCER1G
Gene Name:   Fc fragment of IgE, high affinity I, receptor for; gamma polypeptide
Aliases:   FCRG
RefSeq:   NC_000001.10 NG_013352.1 NT_004487.19
Ensembl:   ENSG00000158869
Entrez:   2207
UniGene:   Hs.433300
Chromosome Mapping:   1q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.800000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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