PrimePCR™ Probe Assay: PPP1R15B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053085
Assay Design:   exonic
Chromosome Location:   1:204375118-204375258question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000367188

Gene Information

PPP1R15B promotes dephosphorylation of the transcription initiation factor EIF2-alpha (EIF2S1; MIM 603907) through recruitment of protein phosphatase-1 (PP1) catalytic subunits (see MIM 176875) (Harding et al. 2009 [PubMed 19181853]).[supplied by OMIM Feb 2010]

Gene Symbol:   PPP1R15B
Gene Name:   protein phosphatase 1, regulatory (inhibitor) subunit 15B
Aliases:   CREP, FLJ14744
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000158615
Entrez:   84919
UniGene:   Hs.304376
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 36.130000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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