PrimePCR™ Probe Assay: AHCYL2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053075
Assay Design:   exonic
Chromosome Location:   7:129029011-129029573question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000325006 ENST00000446544 ENST00000474594 ENST00000490911 ENST00000466993 ENST00000466924 ENST00000531335 ENST00000446212

Gene Information

The protein encoded by this gene acts as a homotetramer and may be involved in the conversion of S-adenosyl-L-homocysteine to L-homocysteine and adenosine. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jun 2011]

Gene Symbol:   AHCYL2
Gene Name:   adenosylhomocysteinase-like 2
Aliases:   ADOHCYASE3, FLJ21719, KIAA0828
RefSeq:   NC_000007.13 NT_007933.15
Ensembl:   ENSG00000158467
Entrez:   23382
UniGene:   Hs.600789
Chromosome Mapping:   7q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 37.000000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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