PrimePCR™ Probe Assay: DUSP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053059
Assay Design:   exonic
Chromosome Location:   2:96809023-96809130question
Amplicon Length:   78
Splice Variants Targeted:   ENST00000356136 ENST00000528420 ENST00000533454 ENST00000531818 ENST00000532130 ENST00000539288 ENST00000397985 ENST00000353706 ENST00000288943

Gene Information

The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK SAPK/JNK p38) which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases different tissue distribution and subcellular localization and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK1 and ERK2 is predominantly expressed in hematopoietic tissues and is localized in the nucleus. [provided by RefSeq Jul 2008]

Gene Symbol:   DUSP2
Gene Name:   dual specificity phosphatase 2
Aliases:   PAC-1, PAC1
RefSeq:   NC_000002.11 NT_022171.15
Ensembl:   ENSG00000158050
Entrez:   1844
UniGene:   Hs.1183
Chromosome Mapping:   2q11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 35.980000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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