PrimePCR™ Probe Assay: SLC37A3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053049
Assay Design:   exonic
Chromosome Location:   7:140035106-140035212question
Amplicon Length:   77
Splice Variants Targeted:   ENST00000340308 ENST00000447932 ENST00000326232 ENST00000469636 ENST00000498469 ENST00000491357 ENST00000485538 ENST00000492027 ENST00000477006

Gene Information

Description Not Available

Gene Symbol:   SLC37A3
Gene Name:   solute carrier family 37 (glycerol-3-phosphate transporter), member 3
Aliases:   FLJ16367, MGC32939
RefSeq:   NC_000007.13 NT_007914.15
Ensembl:   ENSG00000157800
Entrez:   84255
UniGene:   Hs.446021
Chromosome Mapping:   7q34

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 36.240000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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