PrimePCR™ Probe Assay: IL34, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053030
Assay Design:   exonic
Chromosome Location:   16:70693654-70694006question
Amplicon Length:   80
Splice Variants Targeted:   ENST00000288098 ENST00000566361 ENST00000429149

Gene Information

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al. 2008 [PubMed 18467591]).[supplied by OMIM May 2008]

Gene Symbol:   IL34
Gene Name:   interleukin 34
Aliases:   C16orf77, IL-34, MGC34647
RefSeq:   NC_000016.9 NT_010498.15
Ensembl:   ENSG00000157368
Entrez:   146433
UniGene:   Hs.461214
Chromosome Mapping:   16q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.660000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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