PrimePCR™ Probe Assay: PRAMEF15, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PRAMEF15, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PRAMEF15, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0053029
Assay Design:   exonic
Chromosome Location:   1:13648128-13648265question
Amplicon Length:   108
Splice Variants Targeted:   ENST00000267868 ENST00000398117 ENST00000417046 ENST00000064778 ENST00000437584 ENST00000535591 ENST00000235349 ENST00000355096 ENST00000376192 ENST00000376189 ENST00000415464 ENST00000376182 ENST00000414205 ENST00000423177 ENST00000376168 ENST00000376152 ENST00000327795 ENST00000332192 ENST00000330087 ENST00000602960 ENST00000316412 ENST00000372833 ENST00000401694

Gene Information

Description Not Available

Gene Symbol:   PRAMEF15
Gene Name:   PRAME family member 15
Aliases:   PRAMEF9
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000157358
Entrez:   653619
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.540000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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