PrimePCR™ Probe Assay: CD109, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052994
Assay Design:   exonic
Chromosome Location:   6:74534279-74534424question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000287097 ENST00000437994 ENST00000422508

Gene Information

This gene encodes a member of the alpha2-macroglobulin/complement superfamily. The encoded GPI-linked glycoprotein is found on the cell surface of platelets activated T-cells and endothelial cells. The protein binds to and negatively regulates signaling of transforming growth factor beta (TGF-beta). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Apr 2009]

Gene Symbol:   CD109
Gene Name:   CD109 molecule
Aliases:   CPAMD7, DKFZp762L1111, FLJ38569, FLJ41966, RP11-525G3.1
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000156535
Entrez:   135228
UniGene:   Hs.399891
Chromosome Mapping:   6q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 36.400000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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