PrimePCR™ Probe Assay: PHF6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: PHF6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PHF6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052993
Assay Design:   exonic
Chromosome Location:   X:133507484-133511733question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000370803 ENST00000332070 ENST00000370799 ENST00000370800 ENST00000394292 ENST00000416404

Gene Information

This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains indicating a potential role in transcriptional regulation that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS) a disorder characterized by mental retardation epilepsy hypogonadism hypometabolism obesity swelling of subcutaneous tissue of the face narrow palpebral fissures and large ears. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq Jun 2010]

Gene Symbol:   PHF6
Gene Name:   PHD finger protein 6
Aliases:   BFLS, BORJ, MGC14797
RefSeq:   NC_000023.10 NG_008886.1 NT_011786.16
Ensembl:   ENSG00000156531
Entrez:   84295
Chromosome Mapping:   Xq26.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.720000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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