PrimePCR™ Probe Assay: RAET1L, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAET1L, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAET1L, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052960
Assay Design:   exonic
Chromosome Location:   6:150342130-150342262question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000367360 ENST00000479265 ENST00000367351 ENST00000367341 ENST00000286380 ENST00000378954 ENST00000406122 ENST00000402054 ENST00000419554 ENST00000452935 ENST00000539122 ENST00000332337 ENST00000546051 ENST00000370268 ENST00000535861 ENST00000539731 ENST00000449285 ENST00000318504 ENST00000324823 ENST00000594881 ENST00000596469 ENST00000595608 ENST00000599150 ENST00000597697 ENST00000597332

Gene Information

RAET1L belongs to the RAET1 family of major histocompatibility complex (MHC) class I-related genes which are located within a 180-kb cluster on chromosome 6q24.2-q25.3. The REAT1 genes encode glycoproteins that contain extracellular alpha-1 and alpha-2 domains but they lack the membrane proximal Ig-like alpha-3 domain. Most RAET1 glycoproteins are anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (Radosavljevic et al. 2002 [PubMed 11827464]).[supplied by OMIM Mar 2008]

Gene Symbol:   RAET1L
Gene Name:   retinoic acid early transcript 1L
Aliases:   ULBP6
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000155918
Entrez:   154064
Chromosome Mapping:   6q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 36.140000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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