PrimePCR™ Probe Assay: USP25, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: USP25, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: USP25, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052935
Assay Design:   exonic
Chromosome Location:   21:17250750-17250844question
Amplicon Length:   65
Splice Variants Targeted:   ENST00000285679 ENST00000351097 ENST00000285681 ENST00000400183

Gene Information

Ubiquitin (MIM 191339) is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown cell cycle regulation and stress response. Ubiquitin is released from degraded proteins by disassembly of the polyubiquitin chains which is mediated by ubiquitin-specific proteases (USPs) such as USP25 (Valero et al. 1999 [PubMed 10644437]).[supplied by OMIM Mar 2008]

Gene Symbol:   USP25
Gene Name:   ubiquitin specific peptidase 25
Aliases:   USP21
RefSeq:   NC_000021.8 NT_011512.11
Ensembl:   ENSG00000155313
Entrez:   29761
Chromosome Mapping:   21q11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998400
y-intercept 35.470000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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