PrimePCR™ Probe Assay: RAD17, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: RAD17, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: RAD17, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052847
Assay Design:   exonic
Chromosome Location:   5:68709884-68710028question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000576867 ENST00000575406 ENST00000573405 ENST00000571123 ENST00000575651 ENST00000576047 ENST00000573282 ENST00000575476 ENST00000572356 ENST00000572601 ENST00000361732 ENST00000509734 ENST00000354868 ENST00000521422 ENST00000354312 ENST00000345306 ENST00000305138 ENST00000282891 ENST00000380774 ENST00000513214 ENST00000358030 ENST00000374392

Gene Information

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17 a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC) and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene which encode four distinct proteins have been reported. Two pseudogenes located on chromosomes 7 and 13 have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   RAD17
Gene Name:   RAD17 homolog (S. pombe)
Aliases:   CCYC, FLJ41520, HRAD17, R24L, RAD17SP, RAD24
RefSeq:   NC_000005.9 NG_017201.1 NT_006713.15 NW_003315917.1
Ensembl:   ENSG00000152942
Entrez:   5884
Chromosome Mapping:   5q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 39.410000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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