PrimePCR™ Probe Assay: TMEM45B, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052795
Assay Design:   exonic
Chromosome Location:   11:129724532-129724651question
Amplicon Length:   90
Splice Variants Targeted:   ENST00000281441 ENST00000524567

Gene Information

Description Not Available

Gene Symbol:   TMEM45B
Gene Name:   transmembrane protein 45B
Aliases:   FLJ40787
RefSeq:   NC_000011.9 NT_033899.8
Ensembl:   ENSG00000151715
Entrez:   120224
UniGene:   Hs.504301
Chromosome Mapping:   11q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.790000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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