PrimePCR™ Probe Assay: VIPAR, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052776
Assay Design:   exonic
Chromosome Location:   14:77920353-77923465question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000343765 ENST00000553888 ENST00000557658 ENST00000448935 ENST00000556412 ENST00000557466 ENST00000327028

Gene Information

Description Not Available

Gene Symbol:   VIPAR
Gene Name:   VPS33B interacting protein, apical-basolateral polarity regulator
Aliases:   C14orf133, FLJ12707, SPE-39, SPE39, VPS16B, hSPE-39
RefSeq:   NC_000014.8 NT_026437.12 NG_023421.1
Ensembl:   ENSG00000151445
Entrez:   63894
UniGene:   Not Available
Chromosome Mapping:   14q24.3-q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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