PrimePCR™ Probe Assay: PIP4K2A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PIP4K2A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PIP4K2A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052754
Assay Design:   exonic
Chromosome Location:   10:22862272-22880558question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000376573 ENST00000422321 ENST00000545335 ENST00000323883

Gene Information

Phosphatidylinositol-54-bisphosphate the precursor to second messengers of the phosphoinositide signal transduction pathways is thought to be involved in the regulation of secretion cell proliferation differentiation and motility. The protein encoded by this gene is one of a family of enzymes capable of catalyzing the phosphorylation of phosphatidylinositol-5-phosphate on the fourth hydroxyl of the myo-inositol ring to form phosphatidylinositol-54-bisphosphate. The amino acid sequence of this enzyme does not show homology to other kinases but the recombinant protein does exhibit kinase activity. This gene is a member of the phosphatidylinositol-5-phosphate 4-kinase family. [provided by RefSeq Jul 2008]

Gene Symbol:   PIP4K2A
Gene Name:   phosphatidylinositol-5-phosphate 4-kinase, type II, alpha
Aliases:   FLJ13267, PI5P4KA, PIP5K2A, PIP5KII-alpha, PIP5KIIA, PIPK
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000150867
Entrez:   5305
Chromosome Mapping:   10p12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 37.110000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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