PrimePCR™ Probe Assay: PTS, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052753
Assay Design:   exonic
Chromosome Location:   11:112104330-112104450question
Amplicon Length:   91
Splice Variants Targeted:   ENST00000280362 ENST00000525803 ENST00000524931

Gene Information

The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin also known as BH(4) is an essential cofactor and regulator of various enzyme activities including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. [provided by RefSeq Oct 2008]

Gene Symbol:   PTS
Gene Name:   6-pyruvoyltetrahydropterin synthase
Aliases:   FLJ97081, PTPS
RefSeq:   NC_000011.9 NG_008743.1 NT_033899.8
Ensembl:   ENSG00000150787
Entrez:   5805
UniGene:   Hs.503860
Chromosome Mapping:   11q22.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 36.480000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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