PrimePCR™ Probe Assay: KLHL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052739
Assay Design:   exonic
Chromosome Location:   13:70413281-70456494question
Amplicon Length:   64
Splice Variants Targeted:   ENST00000377844 ENST00000545028

Gene Information

The KLHL1 protein belongs to a family of actin-organizing proteins related to Drosophila Kelch (Nemes et al. 2000 [PubMed 10888605]).[supplied by OMIM Feb 2010]

Gene Symbol:   KLHL1
Gene Name:   kelch-like 1 (Drosophila)
Aliases:   FLJ30047, KIAA1490, MRP2
RefSeq:   NG_016173.1 NC_000013.10 NT_024524.14
Ensembl:   ENSG00000150361
Entrez:   57626
UniGene:   Hs.508201
Chromosome Mapping:   13q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 36.300000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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