PrimePCR™ Probe Assay: FERMT3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: FERMT3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FERMT3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052730
Assay Design:   exonic
Chromosome Location:   11:63978807-63979134question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000001008 ENST00000544997 ENST00000345728 ENST00000279227 ENST00000541252 ENST00000401073 ENST00000373481 ENST00000373480 ENST00000400147 ENST00000400149 ENST00000400155 ENST00000400150 ENST00000315677 ENST00000534970 ENST00000539435 ENST00000581699 ENST00000400145 ENST00000581527 ENST00000584874 ENST00000515196

Gene Information

Kindlins are a small family of proteins that mediate protein-protein interactions involved in integrin activation and thereby have a role in cell adhesion migration differentiation and proliferation. The protein encoded by this gene has a key role in the regulation of hemostasis and thrombosis. This protein may also help maintain the membrane skeleton of erythrocytes. Mutations in this gene cause the autosomal recessive leukocyte adhesion deficiency syndrome-III (LAD-III). Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq Jan 2010]

Gene Symbol:   FERMT3
Gene Name:   fermitin family member 3
Aliases:   KIND3, MGC10966, MIG-2, MIG2B, UNC112C, URP2, URP2SF
RefSeq:   NC_000011.9 NT_167190.1 NG_016360.1
Ensembl:   ENSG00000149781
Entrez:   83706
Chromosome Mapping:   11q13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998700
y-intercept 35.760000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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