PrimePCR™ Probe Assay: ADM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052692
Assay Design:   exonic
Chromosome Location:   11:10327894-10328038question
Amplicon Length:   115
Splice Variants Targeted:   ENST00000278175 ENST00000534464 ENST00000530439 ENST00000528655 ENST00000526492 ENST00000525063

Gene Information

Adrenomedullin a hypotensive peptide found in human pheochromocytoma consists of 52 amino acids has 1 intramolecular disulfide bond and shows a slight homology with the calcitonin gene-related peptide. It may function as a hormone in circulation control because it is found in blood in a considerable concentration. The precursor called preproadrenomedullin is 185 amino acids long. By RNA-blot analysis human adrenomedullin mRNA was found to be highly expressed in several tissues. Genomic ADM DNA consists of 4 exons and 3 introns with the 5-prime flanking region containing TATA CAAT and GC boxes. There are also multiple binding sites for activator protein-2 and a cAMP-regulated enhancer element. [provided by RefSeq Jul 2008]

Gene Symbol:   ADM
Gene Name:   adrenomedullin
Aliases:   AM
RefSeq:   NC_000011.9 NT_009237.18
Ensembl:   ENSG00000148926
Entrez:   133
UniGene:   Hs.441047
Chromosome Mapping:   11p15.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.950000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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