PrimePCR™ Probe Assay: ZEB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052679
Assay Design:   exonic
Chromosome Location:   10:31799708-31803532question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000488625 ENST00000542879 ENST00000561304 ENST00000320985 ENST00000559858 ENST00000560721 ENST00000558440 ENST00000424869 ENST00000437844 ENST00000542815 ENST00000558863 ENST00000561212 ENST00000558655 ENST00000561061 ENST00000559496 ENST00000559476 ENST00000557827 ENST00000361642 ENST00000446923

Gene Information

This gene encodes a zinc finger transcription factor. The encoded protein likely plays a role in transcriptional repression of interleukin 2. Mutations in this gene have been associated with posterior polymorphous corneal dystrophy-3 and late-onset Fuchs endothelial corneal dystrophy. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq Mar 2010]

Gene Symbol:   ZEB1
Gene Name:   zinc finger E-box binding homeobox 1
Aliases:   AREB6, BZP, DELTAEF1, FECD6, MGC133261, NIL2A, PPCD3, TCF8, ZFHEP, ZFHX1A
RefSeq:   NC_000010.10 NT_008705.16 NG_017048.1
Ensembl:   ENSG00000148516
Entrez:   6935
UniGene:   Hs.124503
Chromosome Mapping:   10p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.840000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download