PrimePCR™ Probe Assay: PLIN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: PLIN2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: PLIN2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052651
Assay Design:   exonic
Chromosome Location:   9:19126195-19126292question
Amplicon Length:   68
Splice Variants Targeted:   ENST00000276914 ENST00000434144 ENST00000380465 ENST00000380464 ENST00000411567

Gene Information

The protein encoded by this gene belongs to the perilipin family members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material and maybe involved in development and maintenance of adipose tissue. However it is not restricted to adipocytes as previously thought but is found in a wide range of cultured cell lines including fibroblasts endothelial and epithelial cells and tissues such as lactating mammary gland adrenal cortex Sertoli and Leydig cells and hepatocytes in alcoholic liver cirrhosis suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   PLIN2
Gene Name:   perilipin 2
Aliases:   ADFP, ADRP, MGC10598
RefSeq:   NC_000009.11 NT_008413.18
Ensembl:   ENSG00000147872
Entrez:   123
Chromosome Mapping:   9p22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.710000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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