This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: NDUFB11, Human
PrimePCR™ Template for Probe Assay: NDUFB11, Human
NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain the transfer of 2 electrons from NADH to ubiquinone coupled to the translocation of 4 protons across the membrane (Carroll et al. 2002 [PubMed 12381726]).[supplied by OMIM Feb 2009]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.