PrimePCR™ Probe Assay: NDUFB11, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052616
Assay Design:   exonic
Chromosome Location:   X:47004041-47004149question
Amplicon Length:   79
Splice Variants Targeted:   ENST00000377811 ENST00000276062

Gene Information

NDUFB11 is a component of mitochondrial complex I. Complex I catalyzes the first step in the electron transport chain the transfer of 2 electrons from NADH to ubiquinone coupled to the translocation of 4 protons across the membrane (Carroll et al. 2002 [PubMed 12381726]).[supplied by OMIM Feb 2009]

Gene Symbol:   NDUFB11
Gene Name:   NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 11, 17.3kDa
Aliases:   ESSS, FLJ20494, MGC111182, NP17.3, Np15, P17.3
RefSeq:   NC_000023.10 NG_012548.1 NT_079573.4
Ensembl:   ENSG00000147123
Entrez:   54539
UniGene:   Hs.521969
Chromosome Mapping:   Xp11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998300
y-intercept 35.320000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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