PrimePCR™ Probe Assay: HDAC8, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: HDAC8, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HDAC8, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052614
Assay Design:   exonic
Chromosome Location:   X:71792528-71792649question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000379644 ENST00000521576 ENST00000458501 ENST00000297798 ENST00000373583 ENST00000373573 ENST00000373568 ENST00000415409 ENST00000373571 ENST00000439122 ENST00000373560 ENST00000373559 ENST00000373556 ENST00000373554 ENST00000373589 ENST00000429103 ENST00000373561 ENST00000601855 ENST00000600470 ENST00000599422 ENST00000599437 ENST00000595877 ENST00000598854 ENST00000596513 ENST00000601791 ENST00000602209 ENST00000599061 ENST00000595682 ENST00000601811 ENST00000594004

Gene Information

Histones play a critical role in transcriptional regulation cell cycle progression and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Oct 2009]

Gene Symbol:   HDAC8
Gene Name:   histone deacetylase 8
Aliases:   HD8, HDACL1, RPD3
RefSeq:   NC_000023.10 NT_011669.17 NG_015851.1
Ensembl:   ENSG00000147099
Entrez:   55869
Chromosome Mapping:   Xq13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.660000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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