PrimePCR™ Probe Assay: CCT6A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052599
Assay Design:   exonic
Chromosome Location:   7:56131349-56131490question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000275603 ENST00000335503

Gene Information

The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT) also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins including actin and tubulin. Alternate transcriptional splice variants of this gene encoding different isoforms have been characterized. In addition several pseudogenes of this gene have been located. [provided by RefSeq Jun 2010]

Gene Symbol:   CCT6A
Gene Name:   chaperonin containing TCP1, subunit 6A (zeta 1)
Aliases:   CCT-zeta, CCT-zeta-1, CCT6, Cctz, HTR3, MGC126214, MGC126215, MoDP-2, TCP-1-zeta, TCP20, TCPZ, TTCP20
RefSeq:   NC_000007.13 NG_011473.1 NT_033968.6
Ensembl:   ENSG00000146731
Entrez:   908
UniGene:   Hs.82916
Chromosome Mapping:   7p11.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.600000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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