PrimePCR™ Probe Assay: SCML4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052579
Assay Design:   exonic
Chromosome Location:   6:108066206-108066305question
Amplicon Length:   70
Splice Variants Targeted:   ENST00000375248 ENST00000579208 ENST00000314256 ENST00000369022 ENST00000369020 ENST00000440927 ENST00000369021

Gene Information

Description Not Available

Gene Symbol:   SCML4
Gene Name:   sex comb on midleg-like 4 (Drosophila)
Aliases:   FLJ36252, dJ47M23.1
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000146285
Entrez:   256380
UniGene:   Hs.486109
Chromosome Mapping:   6q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 36.080000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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