PrimePCR™ Probe Assay: MUT, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052570
Assay Design:   exonic
Chromosome Location:   6:49398704-49398844question
Amplicon Length:   111
Splice Variants Targeted:   ENST00000274813

Gene Information

This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq Jul 2008]

Gene Symbol:   MUT
Gene Name:   methylmalonyl CoA mutase
Aliases:   MCM
RefSeq:   NC_000006.11 NG_007100.1 NT_007592.15
Ensembl:   ENSG00000146085
Entrez:   4594
UniGene:   Hs.485527
Chromosome Mapping:   6p12.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999800
y-intercept 36.200000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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