PrimePCR™ Probe Assay: ABHD10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052489
Assay Design:   exonic
Chromosome Location:   3:111700797-111705766question
Amplicon Length:   106
Splice Variants Targeted:   ENST00000273359 ENST00000494817 ENST00000534857

Gene Information

Description Not Available

Gene Symbol:   ABHD10
Gene Name:   abhydrolase domain containing 10
Aliases:   FLJ11342
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000144827
Entrez:   55347
UniGene:   Hs.729137
Chromosome Mapping:   3q13.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.870000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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