PrimePCR™ Probe Assay: GPR128, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052488
Assay Design:   exonic
Chromosome Location:   3:100413852-100414001question
Amplicon Length:   120
Splice Variants Targeted:   ENST00000273352 ENST00000475887

Gene Information

Description Not Available

Gene Symbol:   GPR128
Gene Name:   G protein-coupled receptor 128
Aliases:   FLJ14454, FLJ16382, FLJ29035, MGC142011, MGC163260
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000144820
Entrez:   84873
UniGene:   Hs.334511
Chromosome Mapping:   3q12.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 36.200000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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