PrimePCR™ Probe Assay: SLC4A10, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052453
Assay Design:   exonic
Chromosome Location:   2:162735649-162735764question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000415876 ENST00000272716 ENST00000446997 ENST00000421911 ENST00000535165 ENST00000375514

Gene Information

This gene belongs to a small family of sodium-coupled bicarbonate transporters (NCBTs) that regulate the intracellular pH of neurons the secretion of bicarbonate ions across the choroid plexus and the pH of the brain extracellular fluid. The protein encoded by this gene was initially identified as a sodium-driven chloride bicarbonate exchanger (NCBE) though there is now evidence that its sodium/bicarbonate cotransport activity is independent of any chloride ion countertransport under physiological conditions. This gene is now classified as a member A10 of the SLC4 family of transmembrane solute carriers. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq May 2010]

Gene Symbol:   SLC4A10
Gene Name:   solute carrier family 4, sodium bicarbonate transporter, member 10
Aliases:   NBCn2, NCBE
RefSeq:   NC_000002.11 NG_021401.1 NT_005403.17
Ensembl:   ENSG00000144290
Entrez:   57282
UniGene:   Hs.333958
Chromosome Mapping:   2q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.670000
Efficiency 92

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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