PrimePCR™ Probe Assay: PARP1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCEP0052423
Assay Design:   exonic
Chromosome Location:   1:226589925-226590048question
Amplicon Length:   94
Splice Variants Targeted:   ENST00000398988 ENST00000319524 ENST00000509603 ENST00000268763 ENST00000275016 ENST00000328880 ENST00000544745 ENST00000308736 ENST00000354992 ENST00000397394 ENST00000437602 ENST00000452586 ENST00000366794 ENST00000366792 ENST00000366790 ENST00000366791

Gene Information

This gene encodes a chromatin-associated enzyme poly(ADP-ribosyl)transferase which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation proliferation and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition this enzyme may be the site of mutation in Fanconi anemia and may participate in the pathophysiology of type I diabetes. [provided by RefSeq Jul 2008]

Gene Symbol:   PARP1
Gene Name:   poly (ADP-ribose) polymerase 1
Aliases:   ADPRT, ADPRT 1, ADPRT1, PARP, PARP-1, PPOL, pADPRT-1
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000143799
Entrez:   142
UniGene:   Hs.177766
Chromosome Mapping:   1q41-q42

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ Universal SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.550000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download